Hello, I'm a grad student following the Aebersold protocol on SWATH library building: https://www.nature.com/nprot/journal/v10/n3/full/nprot.2015.015.html I'm on Step 37(B)(i) where the Anaconda script spectrast2rsv.py is run to extract the 6 most intense fragment ion peaks from each consensus spectrum to generate a SWATH library.
When I run the script with the recommended options this happens: "C:\TPPdata>python C:\ProgramData\Anaconda2\Scripts\spectrast2tsv.py -1 350,2000 -s b,y -x 1,2 -o 6 -n 6 -p 0.05 -d -e -w swaths.txt -k openswath -a SpecLib_cons _openswath.csv SpecLib_cons.sptxt Traceback (most recent call last): File "C:\ProgramData\Anaconda2\Scripts\spectrast2tsv.py", line 51, in <module> from msproteomicstoolslib.data_structures.aminoacides import Aminoacides ImportError: No module named msproteomicstoolslib.data_structures.aminoacides" In fact, I have installed msproteomicstools and msproteomicstoolslib (which contains data_structures\aminoacides.py, etc.) exists in C:\ProgramData\Anaconda2\Lib\site-packages\. (I am reminded of this fact every time I try to install msproteomicstools in a different location.) What am I missing? Thanks, Steven -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To post to this group, send email to spctools-discuss@googlegroups.com. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.