Hi Debanga, A range as you describe is normal. These values are on a log2 scale, so a SIN delta of 1 roughly corresponds to a 2-fold change +/- the variance. Often the variance can be high for LFQ which makes it challenging to see p < 0.05 when comparing conditions. Without knowing your exact experimental setup, I would suggest sorting from largest to smallest delta SIN and see if those proteins make sense biologically. If you need stat. sig. you may eventually need to move to a targeted or DIA approach with lower variance.
dSIN and SIN are effectively the same, except dSIN includes algorithms which distribute intensity for non unique peptides across the protein group according to the evidence for each protein's existence. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/8d39017c-9848-4a3e-bff1-b0e3338b243a%40googlegroups.com.
