The error message refers to the M[147] modification, which is oxidation of Methionine. Please specify this modification and rerun the tool.
Thanks for using the TPP! -David On Sun, Jan 5, 2020 at 3:13 PM Mehar Un Nissa <meharunnissako...@gmail.com> wrote: > Dear Sir/Madam, > > > I have query related to PTMprophet. > > > I performed comet search for an mzml file and put it for further analysis > as under: > > > > -Using TPP tools Analyse peptides Checked for iprophet proteinprophet > PTMprophet > > > -Mass added for PTMs were: > > > o79.966 Da for Phosphorylation- STY > > > o14.016 Da for Methylation- KR > > > o42.011 Da for Acetylation- K > > > *Query: The analysis is getting failed with a warning message and an error > saying* > > > *“unrecoganized mod taken….* > > > *Error: cannot initialize for sequence: SPRI….”* as in snapshot below. > > > Could you please help me solve this error. > > > Thanks > > > [image: P5.PNG] > > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to spctools-discuss+unsubscr...@googlegroups.com. > To view this discussion on the web visit > https://groups.google.com/d/msgid/spctools-discuss/c99bf3e8-b894-4436-92fb-300615dc0c2a%40googlegroups.com > <https://groups.google.com/d/msgid/spctools-discuss/c99bf3e8-b894-4436-92fb-300615dc0c2a%40googlegroups.com?utm_medium=email&utm_source=footer> > . > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CAGJJY%3D8eLjn2msbiXgc2xKzuE7rp1_aWR6Mev-ozb_K5Mh5C1Q%40mail.gmail.com.