Hi Javan, Many (many) years ago Thermo had a command line executable SEQUEST that BioWorks called to run SEQUEST. Thermo put it inside hidden folders and there were license key issues. You could not copy the EXE to another machine and have it run. That scheme may have persisted for some of the earliest Proteome Discoverer releases.
I do not think that there is still a SEQUEST.EXE associated with Proteome Discoverer. Something made running SEQUEST from the command line break (ancient memory cells are recalling something more...). Maybe it was a transition to C# from C++ and command line parsing issues. I never could get it to work. I gave up trying to run SEQUEST when Comet came out. You can get a 60-day demo version of Proteome Discoverer from Thermo. I do not know if you can create things like pepxml files from PD so that you could post-process the SEQUEST searches with TPP. PD is internally all SQLite3 relational database tables (something like 80 of them). It is either not a very good design for the database tables or just really hard to try and figure out (or both). You can snoop on the SQLite DB with various tools, but a proper schema description would be helpful. Table names and field names are not real transparent. Cheers, Phil On Monday, June 8, 2020 at 3:51:50 PM UTC-7, Jimmy Eng wrote: > > Javan, > > You're not going to be able to run SEQUEST through the TPP unless you've > purchased the software from Thermo and have it installed on the same > computer that's running the TPP. It doesn't come with the TPP itself. And > support for the SEQUEST pipeline option should probably be removed from the > UI unless anyone can confirm that it actually still works. > > On Monday, June 8, 2020 at 1:56:45 PM UTC-7, JAVAN OKENDO wrote: >> >> >> I am getting the above mentioned error when running SEQUEST on TPP. I did >> install the TPP correctly and when I try to run the program it gives an >> error. See the screenshot with the error details >> >> >> >> >> >> [image: Screenshot from 2020-06-08 22-50-26.png] >> >> -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/a9275fb5-b5cd-4f9c-8f97-36789faaeee7o%40googlegroups.com.
