Hello, I am a novice using TPP v5.2.0 Flammagenitus on a workstation (Intel, Xeon, E5-2630 ver.5, 32Gb RAM, win 10). I have successfully run Comet, however, the X!Tandem search is still on command 1 out of 48. Its been more than 56 hours. How much time does it take to complete the search? I am working on 48.Raw files from a Thermo QExactive. The size of each file is approx. 500Mb.
Fixed Mod: Carbamidomethyl on cysteine, Var mod : Oxidation of methionine and N-terminal acetylation. What is the issue with the search? *The params file is: * *<?xml version="1.0" encoding="UTF-8"?>* *-<bioml>* *<note label="list path, default parameters" type="input">D:/TPP/data/params/isb_default_input_kscore.xml</note>* *<note label="spectrum, path" type="input">full_mzXML_filepath</note>* *<note label="output, path" type="input">full_tandem_output_path</note>* *<note label="output, log path" type="input"/>* *<note label="output, sequence path" type="input"/>* *<note label="list path, taxonomy information" type="input">D:/TPP/data/params/taxonomy.xml</note>* *<note label="protein, taxon" type="input">protein_database</note>* *<note label="spectrum, parent monoisotopic mass error minus" type="input">2.0</note>* *<note label="spectrum, parent monoisotopic mass error plus" type="input">4.0</note>* *<note label="spectrum, parent monoisotopic mass error units" type="input">Daltons</note>* *<note label="spectrum, parent monoisotopic mass isotope error" type="input">no</note>* *<note label="residue, modification mass" type="input">57.021464@C</note>* *<note label="residue, potential modification mass" type="input">15.994915@M</note>* *<note label="residue, potential modification motif" type="input"/>* *<note label="protein, N-terminal residue modification mass" type="input"/>* *<note label="protein, C-terminal residue modification mass" type="input"/>* *<note label="protein, cleavage semi" type="input">yes</note>* *<note label="scoring, maximum missed cleavage sites" type="input">2</note>* *<note label="refine" type="input">no</note>* *<note label="refine, maximum valid expectation value" type="input">0.1</note>* *<note label="refine, modification mass" type="input">57.012@C</note>* *<note label="refine, potential modification mass" type="input">15.994915@M</note>* *<note label="refine, potential modification motif" type="input"/>* *<note label="refine, cleavage semi" type="input">yes</note>* *<note label="refine, unanticipated cleavage" type="input">no</note>* *<note label="refine, potential N-terminus modifications" type="input"/>* *<note label="refine, potential C-terminus modifications" type="input"/>* *<note label="refine, point mutations" type="input">no</note>* *<note label="refine, use potential modifications for full refinement" type="input">no</note>* *</bioml>* -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/0be6bd3b-d392-492a-b310-ebf2c62826c0n%40googlegroups.com.
