You would put 1 in that box, since you know you injected 1 ug. On Thursday, December 17, 2020 at 7:14:05 AM UTC-5 meharunn...@gmail.com wrote:
> Thanks Luis. > I started TPP in a new browser and it worked. > Further for my dataset, I need protein abundance to check the comparative > protein expression in each sample. > While using St Peter tool, on what basis the option "*Amount of protein > sample measured*" is decided. > I saw in one of the tutorial that they have taken it as 2.0 micrograms. > For initial MS run, I have injected 1 microgram peptide for obtaining > shotgun data, so what value I should put here for quantification of > proteins present in my sample. > > (Queried option is highlighted in this snapshot) > [image: image.png] > > Looking forward to hearing from you. > Thanks and regards, > > *Mehar Un Nissa* > Research Scholar, > Proteomics Lab. > Department of Biosciences and Bioengineering, > Indian Institute of Technology, Bombay-400076 > > On Wed, Dec 16, 2020 at 9:05 AM 'Luis Mendoza' via spctools-discuss < > spctools...@googlegroups.com> wrote: > >> Hello Mehar, >> It seems that there was an issue with reading your user session cookie -- >> perhaps you followed an old link, reinstalled TPP while logged in, or your >> browser does not support cookies. >> I suggest starting a new browser session and try to run TPP again; let us >> know if the issue persists. Has this happened more than once? >> Cheers, >> --Luis >> >> >> On Mon, Dec 14, 2020 at 8:56 AM Mehar Un Nissa <meharunn...@gmail.com> >> wrote: >> >>> Hello TPP Team, >>> I want to use St. Peter tool in TPP for Label free quantification of >>> some dataset. >>> Once I go to TPP Tools--> Quantify Label Free (MS2), the web page >>> redirected to the login page when I login again some error is coming as >>> under: >>> >>> [image: image.png] >>> >>> Could you please through some light in this regard. >>> Looking forward to hearing from you. >>> Thanks and regards, >>> >>> *Mehar Un Nissa* >>> Research Scholar, >>> Proteomics Lab. >>> Department of Biosciences and Bioengineering, >>> Indian Institute of Technology, Bombay-400076 >>> >>> -- >>> You received this message because you are subscribed to the Google >>> Groups "spctools-discuss" group. >>> To unsubscribe from this group and stop receiving emails from it, send >>> an email to spctools-discu...@googlegroups.com. >>> To view this discussion on the web visit >>> https://groups.google.com/d/msgid/spctools-discuss/CAH%2BQocoJzhCopwYEwOYDH80v164JjdmEoE2brYEH32tv2OzpoQ%40mail.gmail.com >>> >>> <https://groups.google.com/d/msgid/spctools-discuss/CAH%2BQocoJzhCopwYEwOYDH80v164JjdmEoE2brYEH32tv2OzpoQ%40mail.gmail.com?utm_medium=email&utm_source=footer> >>> . >>> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to spctools-discu...@googlegroups.com. >> > To view this discussion on the web visit >> https://groups.google.com/d/msgid/spctools-discuss/CACyS9bqKP9g586_APODu%2BsGmdV5DxxELos-_705JbX9s6%3DgLiw%40mail.gmail.com >> >> <https://groups.google.com/d/msgid/spctools-discuss/CACyS9bqKP9g586_APODu%2BsGmdV5DxxELos-_705JbX9s6%3DgLiw%40mail.gmail.com?utm_medium=email&utm_source=footer> >> . >> > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discuss+unsubscr...@googlegroups.com. To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/77ecf167-581f-4437-b2c0-9cdef570efcen%40googlegroups.com.
