I’m not certain I understand your question. If you’re looking for a
template X!Tandem params file, there should be one in your installation in
the data/params folder. There should be a tandem_params.xml? In the same
folder as the template comet.params? Do you have that?



Eric





*From:* spctools-discuss@googlegroups.com <spctools-discuss@googlegroups.com>
*On Behalf Of *Anju Nagpal
*Sent:* Monday, July 25, 2022 10:33 PM
*To:* spctools-discuss@googlegroups.com
*Subject:* Re: [spctools-discuss] Comet parameters



Can i get the parameter list for Tandem search?

With regards
Anju Nagpal
PhD Scholar
Animal Biotechnology Centre
National Dairy Research Institute, Karnal




On Tue, 26 Jul 2022, 11:01 am 'Eric Deutsch' via spctools-discuss, <
spctools-discuss@googlegroups.com> wrote:

Correct, if you did not use iodoacetamide, then change it to 0.0





*From:* spctools-discuss@googlegroups.com <spctools-discuss@googlegroups.com>
*On Behalf Of *Anju Nagpal
*Sent:* Monday, July 25, 2022 9:35 PM
*To:* spctools-discuss@googlegroups.com
*Subject:* Re: [spctools-discuss] Comet parameters



What if my experiment does not include any alkylation step?

Do I need to change it to 0?



On Mon, Jul 25, 2022 at 8:26 PM 'Eric Deutsch' via spctools-discuss <
spctools-discuss@googlegroups.com> wrote:

Since Cys is almost always alkylated with iodoacetamide in most experiments:



http://www.unimod.org/modifications_view.php?editid1=4





*From:* spctools-discuss@googlegroups.com <spctools-discuss@googlegroups.com>
*On Behalf Of *Anju Nagpal
*Sent:* Monday, July 25, 2022 1:59 AM
*To:* spctools-discuss <spctools-discuss@googlegroups.com>
*Subject:* [spctools-discuss] Comet parameters



Hi,

Why is a default value of 57.021464 is added for cysteine in comet
parameter?

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