Hi David, 

Thank you for such a quick reply! 

I used tpp version 6.2.0 run via a singularity image and I produced the 
table by including the EXCELXX option in proteinprophet. 

Cheers, 

Murielle

On Wednesday, June 28, 2023 at 12:44:03 AM UTC+2 David Shteynberg wrote:

> Hi Murielle,
>
> Thanks for using the TPP and submitting your question here.   When I try 
> the latest version of the TPP and run ProteinProphet through there, the 
> columns exported in my tab separated file from ProteinProphet are different 
> from yours.  Can you please provide a bit more information about how you 
> generated this file and which version of the software you used? 
>
> Cheers,
> -David
>
> On Tue, Jun 27, 2023 at 7:10 AM muriell...@gmail.com <muriell...@gmail.com> 
> wrote:
>
>> Hi all, 
>>
>> I have two questions concerning the output of ProteinProphet :
>>
>> 1) Why do I have some entries for which the total number of peptides is 
>> lower than the number of unique peptides?  (see columns 5 and 6 below, an 
>> extract of my output table)
>>
>> [image: protein prophet output.jpeg]
>> 2) Does the column "total number of peptides" correspond to spectral 
>> counts? 
>>
>> Thank you very much , 
>>
>> Murielle
>>
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