Hi David, 

I tested it with the new version. Now it works well, but CPU usage problem 
still exists. I change threads from 24, 20 to 16 and 8, but the TPP 
searching still uses E-cores. Perhaps it doesn't support P-Cores with Intel 
Hyper-Threading. I am just wondering what CPU you are using for Kojak.exe 
running. 

Thanks again! Anyway, it can still run, though may take a bit longer time. 

Best,
Zeyu 

On Tuesday, May 28, 2024 at 11:17:55 PM UTC-4 David Shteynberg wrote:

> Hello Zeyu,
>
> Sorry I don't know the answer to your question but if it helps I corrected 
> the issue you identified in the previously distributed version compiled 
> with gcc for the TPP distribution installer.
> I placed the gcc compiled version of Kojak.exe here: 
> https://sourceforge.net/projects/sashimi/files/Trans-Proteomic%20Pipeline%20%28TPP%29/TPP%20v7.0%20%28Arafel%29%20rev%20X/Kojak.exe/download
>
> Perhaps this one exhibits a different behavior when running 
> multi-threaded.  In my tests the two version ran nearly at the same speed 
> on my computer.
>
> Cheers,
> -David
>
> On Tue, May 28, 2024 at 7:27 PM Zeyu Wang <wangze...@gmail.com> wrote:
>
>> Hi all,
>>
>> I notice another small issue (maybe this is my win11 problem?): when 
>> running Kojak within TPP, I set the threads to 24 or 28. However, in the 
>> task manager, I notice the main search was always running on exact 
>> CPU16-31, which seem to be E-cores of this 13900K. Did anyone notice 
>> similar problem?   
>>
>> [image: Snipaste_2024-05-28_22-14-14.png]
>> [image: Snipaste_2024-05-28_22-15-57.png]
>>
>> Best,
>> Zeyu 
>>
>>
>>
>>
>>
>> On Monday, May 27, 2024 at 11:12:09 AM UTC-4 Zeyu Wang wrote:
>>
>>> Hi David,
>>>
>>> Thanks! After replacing the current kojak.exe, It seems that now the 
>>> kojak searching can continue without error message.
>>>
>>> Best,
>>> Zeyu 
>>>
>>> On Friday, May 24, 2024 at 9:55:03 PM UTC-4 David Shteynberg wrote:
>>>
>>>> Hello again,
>>>>
>>>> After a bit of digging I found that this is likely caused by a bug in 
>>>> the current TPP compiled and distributed version of Kojak that will have 
>>>> to 
>>>> be corrected for the next release.  Meanwhile you should be able to 
>>>> download and use the official Kojak build available here:  
>>>> https://kojak-ms.systemsbiology.net/download.html
>>>>
>>>>
>>>> This version will require additional .net dependecies!
>>>>
>>>> Please replace the current kojak executable in c:/TPP/bin with the 
>>>> executable from the zip file and try running kojak again.
>>>>
>>>>
>>>> Best of luck and keep us posted to your progress.
>>>>
>>>> -David
>>>>
>>>> On Fri, May 24, 2024, 1:07 PM Zeyu Wang <wangze...@gmail.com> wrote:
>>>>
>>>>> Hi David,
>>>>>
>>>>> Thank you! Here are the two kojak parameter files I tested (the 
>>>>> original and the customized).  Link: Kojak_Troubleshooting 
>>>>> <https://gtvault-my.sharepoint.com/:f:/g/personal/zwang3136_gatech_edu/EnxqUYYUZGNDvCEG8kB-vtsB3Q2GfNvcqaAygJ_0ttBEFA?e=Ub1nub>
>>>>>  .
>>>>>
>>>>> Best,
>>>>> Zeyu 
>>>>>
>>>>>
>>>>>
>>>>>
>>>>>
>>>>>
>>>>>
>>>>> On Friday, May 24, 2024 at 3:47:38 PM UTC-4 David Shteynberg wrote:
>>>>>
>>>>> Hello Zeyu,
>>>>>
>>>>> Thanks for your interest in TPP and Kojak.  Yes we maintain and use 
>>>>> Kojak in the latest release.  To help us troubleshoot this problem, would 
>>>>> you mind sharing the parameter file used in your test?
>>>>>
>>>>> Thank you!
>>>>> -David
>>>>>
>>>>> On Fri, May 24, 2024, 12:28 AM Zeyu Wang <wangze...@gmail.com> wrote:
>>>>>
>>>>> Hi,
>>>>>
>>>>> Are there anyone using Kojak in the TPP v7.0.0 ?  Today I follow the 
>>>>> instruction and try to run a quick test. But at the very beginning, it 
>>>>> reports an error. 
>>>>>
>>>>> "Kojak version 2.1.0, October 16 2023 Copyright Michael Hoopmann, 
>>>>> Institute for Systems Biology Visit http://kojak-ms.org for full 
>>>>> documentation. ****** Begin Kojak Analysis ****** Time at start: Fri May 
>>>>> 24 
>>>>> 02:52:02 2024 Parameter file: c:/TPP/data/params/Kojak_2.conf 12 
>>>>> preconfigured crosslinkers. Reading FASTA database: 
>>>>> c:/TPP/data/dbase/UP000005640_9606_20230222.fasta
>>>>> ...
>>>>>   Total Proteins: 20594
>>>>>   Adding Kojak-generated decoys. New Total Proteins: 41188
>>>>>   5658462 peptides to search (2785870 linkable).
>>>>>
>>>>> Fri May 24 02:52:45 2024 ERROR: 
>>>>> ERROR: cannot open \c:\TPP\data\Test_Kojak\E_ZW_LPS_XL_F10.kojak.txt 
>>>>> for output. Please ensure path and write permissions are correct."
>>>>>
>>>>> Is the error due to the output file path format? Or shall I adjust the 
>>>>> read/write permission of the TPP?
>>>>>
>>>>> Best,
>>>>> Zeyu
>>>>>
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