Fwd from Biofuel. >To: biofuel@yahoogroups.com >From: "roughscience" <[EMAIL PROTECTED]> >Date: Tue, 15 Jul 2003 17:46:03 -0000 >Subject: [biofuel] Re: Prototype Method for 'Salting Out' technique > >--- In biofuel@yahoogroups.com, "chphillips2003" ><[EMAIL PROTECTED]> wrote: > > I don't have a strict method as such, I have just experimenting with > > different ideas based on what little I have learned from my Applied > > Bio-Sciences course at Cardiff University. > > > > Heres basically what I did: > > > > Materials > > > > l00 mL each of ethanol and water > > 10 grams of potassium carbonate > > a few drops of bromothymol blue pH indicator > > a drop of 1 M HCI > > > > Method > > > > Ethanol, water, pH indicator (bromothymol blue) and hydrochloric acid > > are combined in a container. > > The resulting solution is a single-phase and yellow in colour (due to > > the acid). > > Potassium carbonate (which is an base/alkali in solution) is added to > > the mixture which is stoppered and shaken. > > The solution will turn blue and separate into two phases, a colorless > > aqueous and blue organic phase. > > > > > > The HCL and bromothymol blue don't need to be added for the reaction > > to work. The pH indicator just makes it a little easier to distiguish > > between the phases. > > I'm going to repeat this proceedure to try and determine the least > > amount of potassium carbonate required per given volume of > > water/ethanol mix in order to bring about the desired reaction. > > > > Please try this method with different ratios and let me know what you > > find. > > > > Regards > > > > Chris > >I have studied this use of potassium carbonate in a remote past. From >that class I remember that this method cannot be used for obtaining >absolute alcohol. If I remember correctly the max. ethanol >concentration that can be obtained using K2CO3 is 91%. > >Regards, > >Peter.
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