AFAIK, there has not been a bug found that silver cannot kill. It is notable 
that silver does not harm the beneficial flora in the gut. It seems to be 
the perfect antibiotic. IMO, everyone with a weak immune system should be 
either using it routinely or at least have some available for emergencies. I 
have periodontal disease, a sure sign of a weakened immune system.
 Peace, D. Mindock
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
http://www.physorg.com/news7264.html
Study Shows Silver Nanoparticles Attach to HIV-1 virus

In the first-ever study of metal nanoparticles' interaction with HIV-1, 
silver nanoparticles of sizes 1-10nm attached to HIV-1 and prevented the 
virus from bonding to host cells. The study, published in the Journal of 
Nanotechnology, was a joint project between the University of Texas, Austin 
and Mexico Univeristy, Nuevo Leon.
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"Our article opens an important avenue for research," said Miguel Jose 
Yacaman, from University of Texas, Department of Engineering and one of the 
study's authors.

In this study, scientists mixed silver nanoparticles with three different 
capping agents: foamy carbon, poly (PVP), and bovine serum albumin 
(BSA)."Not using a capping agent could result in the synthesis of big 
crystals instead of nanocrystals," explained Yacaman.

Transmission electron microscopy (TEM) showed the silver nanoparticles in 
the foamy carbon matrix were joined together, but an ultrasonic bath in 
deionized water released a significant number of nanoparticles. These 
nanoparticles were of size 16.19 (+-8.69)nm and had the greatest variety of 
shapes, such as icosahedral, decahedral, and elongated.

"Because of the synthesis procedure, the foamy carbon-coated naoparticles 
are more likely to have broad shape distribution," said Yacaman. Scientists 
used the electron beam to release the remainder of the nanoparticles from 
the joined bundle.

For the PVP-coated silver nanoparticles, scientists used glycerine as a 
dissolving agent. These particles were of size 6.53 (+-2.41). In the third 
preparation, scientists used serum albumin, the most common protein in blood 
plasma. The sulfur, oxygen, and nitrogen chemicals in BSA stabilized the 
nanoparticles, which were in the range of 3.12 (+-2.00) nm.

Scientists studied the absorption spectra of the different preparations to 
pinpoint their shapes. "Spherical nanoparticles absorbed in the blue region 
of the spectrum, for example," Yacaman said.

Also, the UV-Visible spectra graphs helped the group determine nanoparticle 
sizes. "The surface plasmon resonance peak wavelength increased with size," 
explained Yacaman.


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Scientists tested, in vitro, each of three silver nanoparticle-preparations 
in HIV-1 cells. Yacaman and his colleagues incubated the samples at 37 C. 
After three hours and 24 hours, respectively, 0% of the cells were living.

The results showed that a silver nanoparticle concentration greater than 25 
ug/mL worked more effectively at inhibiting HIV-1 cells. Plus, the foamy 
carbon was a slightly-better capping agent because of its free surface area. 
Size also played a role since none of the attached nanoparticles were 
greater than 10nm.

Scientists think the nanoparticles bonded through the gp120 glycoprotein 
knobs on HIV-1, using the sulfur residues on the knobs. The spacing between 
the knobs of ~22nm matched the center-to-center nanoparticle spacing.

Although this study shows silver nanoparticles may treat HIV-1, scientists 
need to research this relationship further. "We lack information regarding 
the long-term effects of metal nanoparticles," cautioned Yacaman. Scientists 
are forming a preventive cream for HIV-1, which they will test on humans.

Scientists are also studying other uses for silver nanoparticles. "We're 
testing against other viruses and the 'super bug (Methicillin resistant 
staphylococcus aureus).' Our preliminary results indicate that silver 
nanoparticles can effectively attack other micro-organisms," Yacaman said.

By Syeda Z. Hamdani; Copyright 2005 PhysOrg.com 


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