C57BL/6 <a
href="https://www.creative-bioarray.com/c57bl-6-mouse-bone-marrow-dendritic-cells-csc-c4971j-item-45431.htm";>mouse
bone marrow dendritic cells</a> are derived from the tibias and femurs of
pathogen-free laboratory adult mice. Cells are grown in 100 mm treated tissue
culture dish and incubated in complete growth medium for 5-7 days. Prior to
shipping, cells are detached from dishes and immediately cryo-preserved in
vials. Each vial contains at least 5 x10^6 cells and is delivered frozen. Cells
are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for
expression of markers using antibodies, CD11c by flow cytometry. Cells can be
expanded on a multiwell culture plate ready for experiments under the cell
culture conditions specified by Creative Bioarray.
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