Retroviral vectors promote efficient and stable gene transfer and expression in cultured cells, including primary cells from humans and other animals that are difficult to transfect using other techniques. Transferred genes are integrated at relatively random sites in the host cell genome with minimal alteration of host cell genomic DNA. These properties have resulted in the use of retroviral vectors as markers for cells transplanted into humans, with or without suicide genes to allow destruction of the transplanted cells when necessary, and for human gene therapy.
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