Hello Jialiang--

> 
> I am new to python script of Xplor-nih. I am trying to refine a
> protein structure with PCSs and RDCs. In the structure calculation I
> allow the Da, rh values and tensor orientation varying. But the
> script ends an error, " SystemError: xplor-nih error:
> VarTensor(tm)::calcEnergy: axes are not orthogonal! ". I try to
> refine with just RDCs, it's ok. Then I try to refine with only PCSs,
> it ends the same error. I have no idea. The input tensor parameters
> of PCSs are calculated with Numbat software. What is the reason of
> the error and What should I do to fix the problem?
> 

The error indicates problems with the pseudoatoms associated with the
chi- and alignment tensor(s). This should not happen unless the IVM
configuration is invalid, or the pseudoatoms are manually
manipulated. I would need to see your script to be able to help
further.

best regards--
Charles
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