Hello 陈家良--

> 
> I am refining a protein structure only with PCSs data.All tensor parameters 
> are
> allowed float in the calculation. In theory, the calculation should result in 
> the
> same structures with different starting structures (largely different in some
> regions). But I failed just with a different starting structure (attached
> varytensor-refine file is my script). What should I do?

I think you're saying that your structures do not converge. The first
thing to try is to fix the tensor Da and rhombicity to reasonable
starting values- perhaps suggested by the numbat program. Have you
tried this?

Charles

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