Hi,
I'm trying to use both local and global rdc restraints for RNA structure calculation. The local restraints are coming from smaller constructs of the upper and lower stems that are representative of full length RNA subdomain. I can not seem to use both these rdcs from the small constructs with the imino RDCs from the full length RNA in a protocol similar to the dna_refi refine.py. So I was wondering if rigid body minimization, treating the upper and lower stem as rigid body, fixing the local structure to the local rdcs and applying rigid body minimization that orients these two domains with the iminos from the full length RNA. Would a protocol similar to Wang's work (see below)? Or is there is a better way?
rres2rhft3
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