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Hi--

> We are trying to incorporate multiple dipolar coupling constraints into our
> structure calculations using xplor-nih.  We have a sample input file for
> xplor-nih that has a few force constants (CACO, NCO, HNC, CH) that appear to
> be scaled relative to the N,NH force constant (for example, $k_sani_CACO =
> 0.035*$ksani), but we are unclear about where these force constant estimates
> come from, and how to estimate the missing ones (in particular, HNCA).  Any
> help in this regard would be most appreciated.
> 

This scaling factor takes into account the different gyromagnetic
ratio and bond distance dependence, relative to HNH. The exact formula
(and numbers) can be found in scale_toNH in the python/rdcPotTools.py
file -- if you can read Python. Please let me know if you require
further assistance here.

best regards--

Charles
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