Hi Tom,

Did you try something like

struct @file.psf end
coor @file.pdb
vector show elem (resid) (not known)

to find your problem areas?

--JK

On Sep 12, 2008, at 3:40 PM, Thomas Pochapsky <pochapsk at brandeis.edu>  
wrote:

> Hi,
>
> I generated a multimeric protein structure from a model built in
> PyMOL (painfully, so I don't want to have to do it again!), and
> generated a .psf file with the subunits each generated with the
> segment subroutine:
>
> segment
> name="   A"
> chain
> @toppar:toph19.pep
> coordinates @tet1.pdb
> end
> end
> coor @tet1.pdb
> end
>
>  and each subunit specified with the segment names "    A", "    B"
> etc.   The PDB file I generated has lines in it like:
>
> ATOM      1  N   MET       2      20.811  62.100  33.497  1.00
> 0.00           A
>
> with the label at the end being the chain specifier, and these are
> all appropriate throughout the PDB file.
>
>
> The PSF file shows the following for the same entry:
>
> 1     A 2       MET   N      NH3      -0.100000
> 14.0070               0
>
> Again, each atom appears to have the appropriate label (A or B or
> whatever) immediately preceding the residue number in the psf file.
>
> However, when I try to assign NOEs to atoms in the structure using
> the form shown in eginput mef_dna, (as)
>
> assign ( segid A and resid  48 and name hn ) (segid A and resid 49
> and name hn ) 3.0 0.5 0.5
>
> no atoms are picked and i get an error message:
>
> %NOE-ERR   PROBLEM AT     1   -999.000 -999.000 -999.000 -999.000
> SELRPN:   0 atoms have been selected out of 5322
> SELRPN:  0  atoms have been selected out of 5322
>
> Clearly, my selection statement isn't working, but I have tried every
> variation I can think of of how segid might be constructed, I still
> get the error.  Anybody have any insights?
>
> Thanks,
> Tom Pochapsky
>
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