Hi, If your overall structure is reasonably globular, then the radius of gyration term should work all right. But the expected radius would not double. Rather, you'd calculate it using 2.2 Nres^0.38 where Nres is the total number of residues in both monomers.
Hope this helps. --JK On Sep 11, 2008, at 2:04 PM, "Ramelot, Theresa A. Dr." <ramelota at muohio.edu > wrote: > > > My question is how to use the radius of gyration, collapse term for a > homodimer in the Xplor refinement script. Specifically, I'm > interested in a > domain swapped homodimer that has a very intertwined structure (but > is still > very globular). Do I only "assign" the residues in one subunit and > then use > double the expected radius of gyration? I am also using NCS > symmetry in the > refinement. > > My current format is: > > collapse scale 1.0 > assign (resid 3:66 ) 50.0 10.68 > end > > Will this take the dimer into account, or should I double the expected > radius of gyration to 21.4? > > Thanks, > > Theresa > > Department of Chemistry and Biochemistry, Miami University > > _______________________________________________ > Xplor-nih mailing list > Xplor-nih at nmr.cit.nih.gov > http://dcb.cit.nih.gov/mailman/listinfo/xplor-nih -------------- next part -------------- An HTML attachment was scrubbed... URL: http://dcb.cit.nih.gov/pipermail/xplor-nih/attachments/20080913/d19850fa/attachment.html
