Can anyone help ? ( Apols if these questions are very simple) I am using distance restraints with both upper and lower bounds, starting from extended conformations of proteins ( 100-300 aas) and want to know
1. Can we also WEIGHT the distance restraints INDIVIDUALLY as well as set the bounds ? ( or can we 'only' do this in the anneal program) 2. Can we set negative restraints? ( Or do we simply~ do this by setting long range lower bounds) 3. Is it better to use the anneal program to determine structures from restraints rather than DG ? (we do have false positives ( not many ) and the restraints are sparse, if that helps) -------------- next part -------------- An HTML attachment was scrubbed... URL: http://dcb.cit.nih.gov/pipermail/xplor-nih/attachments/20101213/988f5bf5/attachment.html
