Hi,

When I run a structure refinement, I write outputfiles, either accepted 
(extension *acc.pdb) or rejected (extension *.pdb).
out of 197 structures 141 are accepted, and at structure 142, I get the 
following error message (marked in bold):

  EVALUATE>($filename="result/fold_"+encode($count)+"_acc.pdb")
  EVALUATE: symbol $FILENAME set to "result/fold_142_acc.pdb" (string)
  X-PLOR>   set print-file=$filename end
*%ASSFIL-ERR: no free unit available
  %ASSFIL-ERR: Error accessing file*
  %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
  BOMLEV=    0 reached.  Program execution will be terminated.
  %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
  Subroutine DIE called . Terminating

What is wrong ? Memory problems ? Or is something in the script wrong ?
Any suggestions are welcome.

Thanks,
Ramon

On 13-Feb-10 18:00, xplor-nih-request at nmr.cit.nih.gov wrote:
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>     1. Re: Problems with eginput DNA structure calculations
>        (Charles at Schwieters.org)
>     2. Re: Problems with eginput DNA structure calculations
>        (Charles at Schwieters.org)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 12 Feb 2010 16:15:37 -0500
> From: Charles at Schwieters.org
> Subject: Re: [Xplor-nih] Problems with eginput DNA structure
>       calculations
> To: "DeRose, Eugene (NIH/NIEHS) [C]"<derose at niehs.nih.gov>
> Cc: xplor-nih at nmr.cit.nih.gov,     "Charles at Schwieters.org"
>       <Charles at Schwieters.org>
> Message-ID:<E1Ng2rR-0001BI-DO at tan.cit.nih.gov>
>
> -----BEGIN PGP SIGNED MESSAGE-----
> Hash: SHA1
>
>
> Hello Eugene--
>
>    
>> When I generate the structure file using the old generatedna.inp script in =
>> the tutorial directory, the energies are much better and the structure is n=
>> ot distorted, i.e.,
>>      
> How does that psf compare with eginput/dna_refi/dna_new.psf?
>
> thanks--
> Charles
> -----BEGIN PGP SIGNATURE-----
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>
> iEYEARECAAYFAkt1xPkACgkQPK2zrJwS/larCgCZAYAA0tQZAA6sXI7yayuZS2pt
> xhEAn08J5OYa3k3Ss7DbBfa7vqrj40SI
> =8d4C
> -----END PGP SIGNATURE-----
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 12 Feb 2010 16:32:05 -0500
> From: Charles at Schwieters.org
> Subject: Re: [Xplor-nih] Problems with eginput DNA structure
>       calculations
> To: "DeRose, Eugene (NIH/NIEHS) [C]"<derose at niehs.nih.gov>
> Cc: "xplor-nih at nmr.cit.nih.gov"<xplor-nih at nmr.cit.nih.gov>
> Message-ID:<E1Ng37N-0001NR-Ra at tan.cit.nih.gov>
>
> -----BEGIN PGP SIGNED MESSAGE-----
> Hash: SHA1
>
>
> Hello again Eugene--
>
>    
>> I am trying to use the dna_refi.inp script to compute the structure of the
>> Dickerson dodecamer with some ribose sugar substitutions.  Unfortunately the
>> structures, especially the base geometries are significantly distorted.
>>
>>      
> I think the issue is indeed a topology file mismatch. In
> refine_full.inp, please replace nucleic.top/par with
> nucleic-1.0.top/par, and do this to refine_full.py: add these lines:
>
> protocol.topology['nucleic'] = "nucleic-1.0.top"
> protocol.parameters['nucleic'] = "nucleic-1.0.par"
>
> before the protocol.initParams line. I previously made this change to
> ensemble.py, but forgot to update the other files.
>
> The starting structure is not consistent with the current
> topology/parameters. Please let me know if this clears things up.
>
> best regards--
> Charles
> -----BEGIN PGP SIGNATURE-----
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> iEYEARECAAYFAkt1yNAACgkQPK2zrJwS/laQsQCfZu6NBHVoLXIPfZr5VEB8hrHY
> UuQAn0G+J5GwGuHp3UkyDmjSo99qg8E2
> =v6nX
> -----END PGP SIGNATURE-----
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