Hello 于璐璐--

> 
> I'm trying to use  hydrogen bond constraint in structure
> calculation. So I would like to know what the format of the hydrogen
> bond constraint。The protein is composed of 5 β。

To aid in getting good hydrogen bond geometry, distance restraints for
hydrogen bonds are usually specified in pairs. So, to specify a
backbone hydrogen bond between residues 1 and 20, one would have:

 assign (resid   20 and  name N    )(resid    1 and  name O    ) 3.3 0.8 0.2
 assign (resid   20 and  name HN   )(resid    1 and  name O    ) 2.3 0.8 0.2

I hope this helps.

Charles

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