Hello, 

I'm currently working on a rigid body protocol to calculate ensembles of 
structures for my three-domain protein to optimize the fit with my SAXS data. 
However, I've encountered an issue with the attached script when attempting to 
modify/optimize the weights of each ensemble component, resulting in an error 
message. Could you please help me with the script?

Regarding ensemble modeling with SAXS data, I have some questions:

1.      When determining the ensemble size, is it preferable to use an even 
number, or is the choice of parity irrelevant?
2.      I've noticed discrepancies in the chi2 values between the xray and 
xray-c potentials. Could you clarify the reason for this difference, and which 
value corresponds to the one calculated by calcSAXS or crysol?
3.      Within the script, would it be more advantageous to employ repel and 
torsionDB potentials instead of VDW and RAMA?

I appreciate any guidance you can provide on these matters.
Regards
Diego


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Attachment: saxs_struct_ensemble.py
Description: Binary data

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