Dear xplor mailinglist.
My name is Yoanes Maria Vianney from UGhent. I want to calculate a 30-nt 
DNA-testosterone complex structure. For that I have prepared the basic files 
(PDB, psf, par, restraints, etc) and start to calculate by DGSA using the 
start_structures.py script.
However, in the beginning of the calculation, there is a message in the log 
file showing

AT_Build::buildNode: cycle link found between atoms        31  LIG   C4 and     
   31  LIG   C3
      removing bond.
AT_Build::buildNode: cycle link found between atoms        31  LIG   C9 and     
   31  LIG  C10
      removing bond.
AT_Build::buildNode: cycle link found between atoms        31  LIG  C14 and     
   31  LIG   C8
      removing bond.
AT_Build::buildNode: cycle link found between atoms        31  LIG  C17 and     
   31  LIG  C13
      removing bond.

As such, the testosterone is not in a proper aliphatic ring anymore.
Attached to this email is the start_structures.py script. I am sorry for my 
last email that I forgot to attach it.
What is the problem and what can be modified to prevent the breakage of the 
bond here?
(e.g., we have
try:
    with open('../LIG.psf', 'r') as fh:
        dyn.breakAllBondsIn("not resname LIG")
In the IVM but it still remove the bond in the LIG.

Thank you in advance.

Best regards,
Yoanes Maria Vianney

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Attachment: start_structures.py
Description: start_structures.py

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