Hi,
If your overall structure is reasonably globular, then the radius of
gyration term should work all right. But the expected radius would not
double. Rather, you'd calculate it using 2.2 Nres^0.38 where Nres is
the total number of residues in both monomers.
Hope this helps.
--JK
On Sep 11, 2008, at 2:04 PM, "Ramelot, Theresa A. Dr." <[EMAIL PROTECTED]
> wrote:
My question is how to use the radius of gyration, collapse term for a
homodimer in the Xplor refinement script. Specifically, I'm
interested in a
domain swapped homodimer that has a very intertwined structure (but
is still
very globular). Do I only "assign" the residues in one subunit and
then use
double the expected radius of gyration? I am also using NCS
symmetry in the
refinement.
My current format is:
collapse scale 1.0
assign (resid 3:66 ) 50.0 10.68
end
Will this take the dimer into account, or should I double the expected
radius of gyration to 21.4?
Thanks,
Theresa
Department of Chemistry and Biochemistry, Miami University
_______________________________________________
Xplor-nih mailing list
[email protected]
http://dcb.cit.nih.gov/mailman/listinfo/xplor-nih
_______________________________________________
Xplor-nih mailing list
[email protected]
http://dcb.cit.nih.gov/mailman/listinfo/xplor-nih
