Hello Jakob--

>
> I would like to use xplor-NIH, if possible, to calculate a structure
> for a supramolecular complex using constraints from both NMR and
> cryo-EM.

Very good!

>
> Cryo-EM provides density constraints for all points in space. Is
> this possible with xplor-NIH? Can I find a template script for this?
> What kind of format would be required for the density file?

We have a potential term which should be appropriate for this use:
probDistPot, which is probably best set up using probDistPotTools. The
only example of its use currently distributed is in
python/tests/probDistTest.py in the Xplor-NIH distribution. This term
takes an AtomDensity object
(http://nmr.cit.nih.gov/xplor-nih/doc/current/python/ref/atomDensity.html)

which should be able to read in CCP4-formatted maps.

Now, we have never used the term for this exact purpose, so there may
be bugs. Moreover, this functionality is not particularly well
organized or documented. In short, this will likely require a bit of
work, which I would gladly provide.

best regards--
Charles
--
Charles Schwieters     email:   [email protected]
                       www:     http://schwieters.org/cds
phone: (301) 402-4914  PGP key: http://schwieters.org/cds/pgp.txt

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