Hi Bala No, this is only for DNA sequence comparisons.
Regards Tim On 7/11/06 12:02, "BALASUBRAMANIAN GANESAN" <[EMAIL PROTECTED]> wrote: > Dear Tim/Julian/users > Is it also possible to use protein sequence comparisons using this same > approach? > Regards > BALA. >> ===== Original Message From Julian Parkhill <[EMAIL PROTECTED]> ===== >> Bala, >> >> ACT cannot reconstruct the positional information for the separate >> sequences within a multiple FASTA file; BLAST reports only the local >> coordinates for the matches. What you need to do is: >> >> 1) Concatenate all the mFASTA sequences into a single sequence for >> each genome (you can do this from Artemis, using the "Write; all >> bases" menu) >> >> 2) Do the blast comparison with the concatenated sequences >> >> 3) Load the original multiple FASTA files into ACT, but use the >> comparison file from the concatenated sequences. You should see the >> contigs represented by alternating dark/light brown features, plus >> the matches for the whole sequences. >> >> For the other problem; the set cutoffs only remain for as long as the >> dialog window is open. Closing the dialog window resets the cutoffs. >> >> yours, >> >> Julian. >> > _______________________________________________ Artemis-users mailing list Artemis-users@sanger.ac.uk http://lists.sanger.ac.uk/mailman/listinfo/artemis-users
