Hi again,

> Are you sure that everything is correctly linked? Use the "Experiment
> overview" to find out. It should find
> 
> 1) The raw bioassay(s) that doesn't have the annotation.
> 2) The raw bioassays that has inherited the same annotation from
> multiple parents.
> 
> Maybe it is as simple as re-linking one or more parents from the raw
> bioassays in 2) to the raw bioassays in 1).
> 
> /Nicklas


No, I have checked and things seem correctly linked, but all the raw
bioassays have inherited two factor values for the experimental factor
(and this is indeed being flagged up by the experiment overview). 

The data I am trying to import is just made up example data for a
fictitious two-colour experiment (see below). I have 4 raw bioassays,
two from a mutant, two from a wildtype, but both of them inherit both
"mutant" and "wildtype" as their factor value, although they are
correctly linked upwards to their respective biosource.

Could the problem be coming from the fact that in two-colour experiments
there is not a straight, one-to-one line of inheritance from the
BioSource to the RawBioAssay? I have a suspicion that the problem comes
from having two labelled extracts (which come from two different
biosources) used in the same hybridization, which may then pass these
both on downstream to the raw bioassay.

Cheers

Micha

==============

Raw data follows:

RawBioAssay     ArrayBatch      ArraySlide      Scan    Hybridization
LabeledExtract  Dye     Extract Sample  BioSource       StrainOrLine
RBA1     batch 1         slide 1        scan  hyb1       hyb1
mutant1.s1.e1.le1       cy5     mutant1.s1.e1   mutant1.s1      mutant1
mutant
RBA2     batch 1         slide 1        scan  hyb1       hyb1
wildtype1.s1.e1.le1     cy3     wildtype1.s1.e1 wildtype1.s1
wildtype1       wildtype
RBA3     batch 1         slide 2        scan  hyb2       hyb2
mutant1.s1.e1.le1       cy5     mutant1.s1.e1   mutant1.s1      mutant1
mutant
RBA4     batch 1         slide 2        scan  hyb2       hyb2
wildtype1.s1.e1.le1     cy3     wildtype1.s1.e1 wildtype1.s1
wildtype1       wildtype

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