On 05/11/2011 11:51 AM, Ivan Gregoretti wrote:
Hello Martin,
When I do annotation(Data) I get the response
"mouse4302"
What would you do now?
maybe
library(annotate)
pkg <- annPkgName(annotation(Data))
ok <- require(pkg, character.only=TRUE)
if (!ok)
stop(pkg, " not found; install using biocLite?")
Thank you,
Ivan
Ivan Gregoretti, PhD
National Institute of Diabetes and Digestive and Kidney Diseases
National Institutes of Health
5 Memorial Dr, Building 5, Room 205.
Bethesda, MD 20892. USA.
Phone: 1-301-496-1016 and 1-301-496-1592
Fax: 1-301-496-9878
On Wed, May 11, 2011 at 1:29 PM, Martin Morgan<mtmor...@fhcrc.org> wrote:
On 05/11/2011 08:54 AM, Sean Davis wrote:
On Wed, May 11, 2011 at 11:38 AM, Ivan
Gregoretti<ivang...@gmail.com>wrote:
Hello Jim,
First, thank you for responding.
Indeed, the issue is the request to generate a personal library.
I use a wide variety of Bioconductor packages, from the most canonical
ones like ShortRead to more obscure ones like cosmo, however, no
function ever asked me to create a personal library as a condition for
execution.
Do you regularly encounter functions that make such demand? I am curious.
Hi, Ivan.
This request is from R, itself, and not from the affy package or any other
Actually, rma needs the cdf package corresponding to the chip, and calls
getCdfInfo and eventually cdfFromBioC if it is not installed. This is
apparently determined by getOption("BioC")$affy$probesloc, but this is not
well-documented (which is an understatment). A work-around would make sure
the package (from annotation(Data)) is installed before calling rma(), and
then you'd at least have more control over what to do if prompted.
just.rma is a (much) more memory efficient approach if the goal is to arrive
directly at an ExpressionSet without needing the AffyBatch.
Martin
package. If you haven't seen this before, it was probably because a user
Library existed previously (you are asked only the first time it is
needed)
or because the default system package location was writeable by you. See
the R manuals for details on library locations if you want more detail.
Sean
Thank you,
Ivan
PS: By the way, cosmo rocks.
Ivan Gregoretti, PhD
National Institute of Diabetes and Digestive and Kidney Diseases
National Institutes of Health
5 Memorial Dr, Building 5, Room 205.
Bethesda, MD 20892. USA.
Phone: 1-301-496-1016 and 1-301-496-1592
Fax: 1-301-496-9878
On Tue, May 10, 2011 at 7:30 PM, James W. MacDonald
<jmac...@med.umich.edu> wrote:
Hi Ivan,
On 5/10/2011 4:41 PM, Ivan Gregoretti wrote:
There seems to be a dependency error that comes up when running an
analysis with affy. It only takes three commands.
###################################################################
library(affy)
Data<- ReadAffy(file="/home/johndoe/GSM492799.CEL.gz")
eset<- rma(Data)
Warning in install.packages(cdfname, lib = lib, repos =
Biobase:::biocReposList(), :
'lib = "/usr/local/lib64/R/library"' is not writable
Would you like to create a personal library
~/R/x86_64-unknown-linux-gnu-library/2.14
to install packages into? (y/n)
###################################################################
Is this error reproducible by others?
I don't see an error here. But perhaps you are referring to the warning?
I'm not sure how it could be made clearer, however. The gist is that you
are
running R as non-root (as you should), and therefore don't have write
access
to the /usr/local/lib64/R/library, (which you should not), so R is
stating
that fact and asking you if you want it to create a personal library in
your
home directory, where in future it will look for any packages that
aren't
found in the 'usual' place.
So, long story short, if you had simply typed a 'y' after that warning,
you
would have got the package downloaded and gone about your business.
Best,
Jim
Thank you
Ivan
sessionInfo()
R version 2.14.0 Under development (unstable) (2011-04-14 r55450)
Platform: x86_64-unknown-linux-gnu (64-bit)
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] affy_1.31.1 Biobase_2.13.1
loaded via a namespace (and not attached):
[1] affyio_1.21.0 preprocessCore_1.15.0 tools_2.14.0
Ivan Gregoretti, PhD
National Institute of Diabetes and Digestive and Kidney Diseases
National Institutes of Health
5 Memorial Dr, Building 5, Room 205.
Bethesda, MD 20892. USA.
Phone: 1-301-496-1016 and 1-301-496-1592
Fax: 1-301-496-9878
_______________________________________________
Bioc-sig-sequencing mailing list
Bioc-sig-sequencing@r-project.org
https://stat.ethz.ch/mailman/listinfo/bioc-sig-sequencing
--
James W. MacDonald, M.S.
Biostatistician
Douglas Lab
University of Michigan
Department of Human Genetics
5912 Buhl
1241 E. Catherine St.
Ann Arbor MI 48109-5618
734-615-7826
**********************************************************
Electronic Mail is not secure, may not be read every day, and should not
be
used for urgent or sensitive issues
_______________________________________________
Bioc-sig-sequencing mailing list
Bioc-sig-sequencing@r-project.org
https://stat.ethz.ch/mailman/listinfo/bioc-sig-sequencing
[[alternative HTML version deleted]]
_______________________________________________
Bioc-sig-sequencing mailing list
Bioc-sig-sequencing@r-project.org
https://stat.ethz.ch/mailman/listinfo/bioc-sig-sequencing
--
Computational Biology
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109
Location: M1-B861
Telephone: 206 667-2793
--
Computational Biology
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109
Location: M1-B861
Telephone: 206 667-2793
_______________________________________________
Bioc-sig-sequencing mailing list
Bioc-sig-sequencing@r-project.org
https://stat.ethz.ch/mailman/listinfo/bioc-sig-sequencing
