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Hi Zhi,
you could do that with molrep , by specifying MODEL2 as your fixed input
model and MODEL1 as your model to search the second subunit (MODEL2 will
not be moved). If you want to use phases just add to the LABIN command
which phases to use. Check Alexei's webpage for a more recent version of
molrep .
http://www.ysbl.york.ac.uk/~alexei/
Jürgen
ZhiYi Wei wrote:
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hi, all
I want to do a phased MR, but I can not figure out how to do it.
Some backgrounds:
the protein containing two subunits;
a poor SAD-phasing map;
got a correct MR of one subunit.
A phased MR can be used to solve the position of the other subunit. The question
is that how to fix the correct subunit position in a phased MR, which program
can
do that?
Thanks alot.
------------------------------
Zhiyi Wei
---------------------------------------------
Institute of Biophysics, Chinese Academy of Sciences
15 Datun Road, Chaoyang District
Beijing, P.R.China, 100101
---------------------------------------------
School of Life Sciences,
University of Science & Technology of China,
Hefei, Anhui, P.R.China, 230026
Tel:86-551-3607334
Fax:86-551-3607154
--
--------------------------------------------
Jürgen Bosch
University of Washington
STRUCTURAL GENOMICS OF PATHOGENIC PROTOZOA
Health Science Building
Dept. of Biochemistry, K-418
Box 357742
1705 NE Pacific Street
Seattle, WA 98195-7742
Phone: 206-616-4542
FAX: 206-685-7002
Web: www.sgpp.org
