Hi,
Sorry for this off-topic. I have a highly basic protein, whose pI is over 11. I don't know if its pI causes the difference of expression in E.coli pET system. Anyone has general experience of expressing such proteins? Thanks!
Yadong Yu
LCMN/NICHD/NIH
Bldg 35, Room 3B 1006
35 Lincoln Drive
Bethesda MD 20892
(301)496-9347
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- [ccp4bb]: expression of highly basic protein Yadong Yu
- Re: [ccp4bb]: expression of highly basic protein Ashley Buckle
- Re: [ccp4bb]: expression of highly basic protein Artem Evdokimov
