Re: [ccp4bb]: expression of highly basic protein

[Ashley Buckle]

Have a look at our REFOLD database - there are a few cases where the pI >11, and it looks like one of them uses the pET system: http://refold.med.monash.edu.au/results.php?search=search&cboProteinOp=1&pi_op=9&pi=11 cheers ashley On 14/03/2006, at 10:38 AM, Yadong Yu wrote: Hi,  Sorry for this off-topic. I have a highly basic protein, whose pI is over 11. I don't know if its pI causes the difference of _expression_ in E.coli pET system. Anyone has general experience of expressing such proteins? Thanks! Yadong Yu LCMN/NICHD/NIH Bldg 35, Room 3B 1006  35 Lincoln Drive  Bethesda MD 20892 (301)496-9347 Fax (301)496-2396 [EMAIL PROTECTED] Ashley Buckle Ph.D Research Fellow The Department of Biochemistry and Molecular Biology  School of Biomedical Sciences, Faculty of Medicine & Victorian Bioinformatics Consortium (VBC) Monash University, Clayton, Vic 3800 Australia http://vbc.med.monash.edu.au/~abuckle Tel: (613) 9905 3781 (office) Tel: (613) 9905 1653 (lab) Mobile: 0400 206069 Fax : (613) 9905 4699