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Hi Jie Liu,
Are the two helices in your coiled coil related by a two-fold
crystallographic axis.
Crystallographic symmetry axes are notorious for showing strange
electron density
artifacts.
In most cases crystallographic axes do not fall into the center of
our molecules, so
we typically do not pay any attention to these "on-axis" density
artifacts, but they are
almost always present and usually cannot be interpreted in terms of a
molecular structure.
Good luck,
Ulrich
--
Ulrich K. Genick
Assistant Professor
Department of Biochemistry
Brandeis University, MS009
Waltham, MA, 02454
Room Kosow 108
Phone 781-736 2304
Fax 781-736 2349
Email [EMAIL PROTECTED]
Do you have a crystallographic two-fold axis running through the
center of your
On Mar 13, 2006, at 6:39 PM, Jie Liu wrote:
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Dear you all
Sorry for the off-topic question.
I saw long continous string of difference electron density inside
the hydrophobic core of my coiled-coil structure running almost
the full length of the helices. There are only sodium citrate (pH5.6)
and hexane-1,6-diol in the crystallization solution. I am wondering
whether it is possbile that this density corresponds to polymerized
hexanediol? Did anyone have this experience before or could anyone
point to a reference?
Your kind help will be greatly appreciated.
Jie Liu
