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Hi Jie Liu,
Is it possible that you have used detergent in the protein
purification process or crystallization? By chance does this protein
bind to long hydrophobic molecules like steroids or fatty acids? I
had a similar experience with Cholesterol esterase, where it binds to
a detergent molecule in the active site. Acta Crystallographica,
2003, D59, 50-56.
Good luck!
Anthony
On Mar 13, 2006, at 3:39 PM, Jie Liu wrote:
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Dear you all
Sorry for the off-topic question.
I saw long continous string of difference electron density inside
the hydrophobic core of my coiled-coil structure running almost
the full length of the helices. There are only sodium citrate (pH5.6)
and hexane-1,6-diol in the crystallization solution. I am wondering
whether it is possbile that this density corresponds to polymerized
hexanediol? Did anyone have this experience before or could anyone
point to a reference?
Your kind help will be greatly appreciated.
Jie Liu
