All:
The last question on _expression_ of a
highly basic protein brought up a question I wanted to post. We are
working with a highly positively charged protein (numerous Lys and Arg residues
on the surface) and are having no trouble expressing it, but are having trouble
crystallizing it. We see some small crystals that appear to be protein
crystals from a high-pH condition (as you would expect for a basic protein), but
are having trouble optimizing the conditions. I was wondering: has anyone
worked out a crystallization screen
(or mini-screen) for highly basic proteins? If so, can someone provide me
with a reference ?
Thanks.
-Dirk
Dirksen
Bussiere, Ph.D.
Associate
Director
Computational
Chemistry and Structural Chemistry
Chiron
Corporation
4560 Horton
Street, M/S 4.4
Emeryville,
CA.
94608
Phone:
510-923-2114
Fax:
510-923-5550
e-mail: [EMAIL PROTECTED]
