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Although a 33 fold excess seems like it should be enough for sufficient
vapour diffusion to occur, does anyone who has experience with 96 well plates
say otherwise??
I use these for every initial screen (along with microbatch), and I'm
quite happy with the results. I've also used 1uL:1uL with 50uL wells, and
that seems to be fine as well. My coworkers have had similar success.
I would definitely say that reproducing the crystals in a more standard
format (i.e. 24-well sitting/hanging drop trays) doesn't always work as
expected, but I've had this problem regardless of screen format. (I
suspect as more and more people use robots this will continue to be an
issue.)
My only real complaints are:
- our 8-channel pipet isn't very accurate, which of course makes
reproducibility harder.
- those trays are awfully hard to extract crystals from.
- They also tend to dry up sooner.
- it can be hard to identify crystals, depending on the shape of the
mini-well where the drop sits. crystals often stick to the side of the
mini-well, at least in the trays I use most often now.
-Nat
