*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Hi Jin,
you'll need to run mapman on the EM map and write it out as an EZD map,
then edit the origin, so that the center of mass is no longer in the
origin. Read it back into mapman and write it out again as ccp4 map.
Then you need to change the grid size as SFALL specifies it. That should
be all what you have to do.
Small hint, change the size of your EM map by -10% to +10% in steps of
e.g. 2% and then run your Molecular replacement with 10 different models.
Good luck,
Juergen
Jin K. Yang wrote:
*** For details on how to be removed from this list visit the ***
*** CCP4 home page http://www.ccp4.ac.uk ***
Hi all,
I'm trying to get Fc from EM map for the use of MR search model.
The EM map is now in the P1 cell which has 64 grid points on each axes, and is
in the box [-32 to 31, -32 to 31, -32 to 31].
When I tried SFALL to get Fc from this map, the SFALL compained as below in the
last part of the logfile.
Check map header agrees with fixed requirements for SFcalc for this spacegroup.
Check KV1 KV2 KU1 KU2 Ksec,Msec - IXmin IXmax..
-32 31 -32 31 -32 64 0 63 0 63 0 63
<B><FONT COLOR="#FF0000"><!--SUMMARY_BEGIN-->
SFALL: **** Fatal disagreement between input info and map header
It looks to me that my map should be located in the [0 63, 0 63, 0 63] which is
an asu defined in SFALL.
The manual of SFALL also looks like indicating this point.
The EM density has a shape of a triangular disk whose center is located in the
origin of the coordiates system.
How can I move this density into the box [0 63, 0 63, 0 63] and update map
header information accordingly so that SFALL can read my density ?
Thanks in advance.
JK
--
Jürgen Bosch
Howard Hughes Medical Institute and
University of Washington
Dept. of Biochemistry, K-418
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4542
FAX: +1-206-685-7002
