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Hi Peter,
The crystals for nucleosomes (protein-DNA) always exhibit diffraction
anisotropy (if that's what you are referring to). As painful as it was,
it was not a severe impediment for integration and/or refinement with
the few precious decent crystals.
Here's some interesting stuff I recently came across with respect to
treating data with severe diffraction anisotropy - basically applying
ellipsoidal resolution cutoffs and anisotropic scaling.
Might help to check this out.
http://nihserver.mbi.ucla.edu/anisoscale/
Hope that's a lead.
Raji
W.M. B. wrote:
Hi there:
I am crystallizing a protein-dna complex. The diffraction from one
direction is very good. when the crystal turns 90 degree, it has
streaking diffraction. I can't get a very good dataset in order to
solve the structure.
The crystal is good-looking like the cubic ice. I'm tring to
crystalize the complex from different conditions. Is there any other
way to improve the diffraction? Any suggestion would be greatly
appreciated.
Peter
--
Raji Edayathumangalam
Postdoctoral Fellow
The Rockefeller University
New York, NY 10021
