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Dear Peter, I assume that you are collecting data under cryo-conditions? Have you also tried collected two images at 90 degrees at room temperature (best to do this on a home source!)? This will tell you whether it is the crystals themselves at fault or if it is just cryoprotectant damaging the crystals. Also, how 'streaky' are the spots, are they just a blur, or is there an obvious center peak in the spots. You may still be able to collect some usable data in the second case. Andy On Tue, 2006-10-17 at 16:56 -0400, W.M. B. wrote: > Hi there: > > I am crystallizing a protein-dna complex. The diffraction from one > direction is very good. when the crystal turns 90 degree, it has > streaking diffraction. I can't get a very good dataset in order to > solve the structure. > > The crystal is good-looking like the cubic ice. I'm tring to > crystalize the complex from different conditions. Is there any other > way to improve the diffraction? Any suggestion would be greatly > appreciated. > > Peter > > > > -- Cat, n.: Lapwarmer with built-in buzzer. +----------------------------------------------------------------------+ | Andy Purkiss-Trew, School of Crystallography,Birkbeck College,London | | E-mail [EMAIL PROTECTED] | | Phone 020 7631 6869 (Work) or 07763 490 360 (Mobile) | +----------------------------------------------------------------------+
