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COULD SOMEONE from the ccp4bb PLEASE DELETE M.K. SWAN's PRIVILEGE TO ENTER THE bb. I CANNOT STAND THAT UNBEARABLE EMAIL ANYMORE. Marius > Hi All, > I have a question about processing in HKL2000. > I have a dataset which looks like it will go out to 2.5A but the > problem > is that when I have integrated the 360 frames (180degrees) to 2.4A I > get > a large number of rejects on scaling which exceeds the 50000 limit. > So > is there any way to increase this limit or another method of reducing > the number of rejects? > I can get scaling to work if I either cut the resolution down to 3.0 > or > lower but alternatively if I exclude alot of frames and leave say 90 > then the rejects are below 50000 and the scaling produces data that > looks good to at least 2.6-2.5A according to the I/sigma and an > Rmerge > <0.5 in the final shell, however the completeness suffers due to the > low > number of frames. > I should also say that I am suspicious that one of the reasons for > all > the rejects might be that in the centre of the pattern the spots are > of > a good radial shape but towards the edge they become more elongated. > I > tried using the elliptical spot feature of HKL2000 but this doesn't > seem > to be ideal as the spots are not always elongated in the same > direction. > Any thoughts or suggestions would be much appreciated, > > Mike Swan. PD Dr. habil. Marius Schmidt Physikdepartment E17 Technische Universitaet Muenchen James Franck Strasse 85747 Garching/Germany email: [EMAIL PROTECTED] phone: +49-(0)89-2891-2550 fax: +49-(0)89-2891-2548
