hi all
first of all i apologise for asking a non CCP4 question.
i m trying to solve a structure by MIR using Solve/Resolve. i have native
data up to 1.8A but the data quality for derivatives (Pt, Hg, Au and Sm) is
not very food in terms of resolution and occupancies. due to this i have
calculated patterson at 3.3A with a mean fig of merits: 0.43. i m also using
anomalous data for patterson calculation. Mean FOM after density
modification using resolve at 3.7A is 0.67. i cant further improve the
FOM using NCS although there r two molecules in asu, becz no NCS relation
exist between the heavy atom sites detected. since i m working at very low
resolution, automated model building is placing either ala or gly. Is there
anything that i can try to further improve theFOM n secondly if i cant
further improve the FOM, how should i proceed in solving structure with a
partially build model for main chain.
thanx in advance
vineet gaur
