Dear Mathews,
we were successful in crystallizing two ferredoxins under strict anaerobic
conditions using the much cheaper solution of a glove bag and filling it
with argon. I guess that in both cases (box and bag) the thickness of the
gloves is a problem especially if you are dealing with cover slides. The
glove bag occupies also much less space however its major problem was the
very bad visualization of the inner space and finally we added a plexiglas
window on the bag. Needless to say how essential is not to forget anything
outside the bag/box before starting the crystallization set up.
Finally you can also consider the possibility to use a crystallization
robot. In some of them it is possible to create anaerobic conditions using
argon.
good luck
Nikos
*************************************
Nikos Pinotsis, PhD
EMBL-Hamburg, c/o DESY
Notkestr. 85, Geb. 25A
22603 Hamburg, Germany
Phone : +49 40 89902144
Fax : +49 40 89902149
e-mail : [EMAIL PROTECTED]
*************************************
----- Original Message -----
From: "Mathews, Irimpan" <[EMAIL PROTECTED]>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Friday, 23 February, 2007 3:03 AM
Subject: [ccp4bb] Question about glove boxes for protein crystallization
Dear Friends,
Sorry for the non CCP4 question. We are planning to purchase a small glove
box to setup crystallization trays under anaerobic conditions. If you have
used glove boxes for crystallization, would you please give me some idea?
We are thinking of getting the 815 series from Plas-labs (link below).
http://www.plas-labs.com/
Thank you very much,
Mathews
Ps: If others are interested, I will post a summary.