Hi All!
I have been trying to screen for my protein crystals, from the
crystals grown in 0.5 M Ammonium Sulphate, 1.0M Lithium Sulphate
Monohydrate in 0.1 M TriSodium Citrate Buffer dihydrate Buffer at pH 5.6.
Two different kinds of crystals observed: rod shaped and thin platy
ones. Whenever I am trying to collect data from the rod shaped ones I am
getting dominantly salt patterns but also spots at 15 A resolution bin
repeatedly, where some of the spots very much look like from protein!
what can be the reason for this? if anyone has come across similar
situation please help.
with regards,
Sreeram Mahesh
Research Student
Prof S Ramakumar's lab
PHYSICS department
IISc Bangalore-560 012.
ph:080-2293 2718.
mobile: 9241145183.
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