Dear Sajid,
the problem with all oils is that they allow some small evaporation that
is why your drops dry out. You can set up your crystal drops over a
reservoir (you use microbridges) which will be the same as your
crystallization media and your drops won't dried out.
best of luck
Demetres
sajid akthar wrote:
Hi All
I'm getting clusters of crystals (tiny and long needles) and I'm trying to get
good single crystals. Even I'm getting the same result at low salt
concentrations. I reduced the temperature to slow down the evaporation, still
no change. So I'm trying with mineral oil. Here I'm getting somewhat thicker
needles and the number of needles is reduced. But my drop seems to be dried out
in two or three days (I dont think there is a sealing problem). Can anyone
suggest me to overcome this and to get good crystals.
Thankz in advance
Sajid
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Demetres D. Leonidas, Ph.D.
Structural Biology & Chemistry Group
Institute of Organic and Pharmaceutical Chemistry
The National Hellenic Research Foundation
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