Hi Vijay,If it is C-terminal degradation, fusing the His-tag to the C-terminus may help you get rid of it.
Wataru Kagawa ######################################### Wataru Kagawa, Ph. D. Research Scientist Protein Research Group RIKEN (Physical and Chemical Research Institute) W221, West Research Bldg. 1-7-22 Suehiro-cho, Tsurumi-ku Yokohama, Japan 230-0045 tel. 045-503-9206 fax. 045-503-9201 #########################################
