Jacob, Whether the calbiochem/anatrace catalogues will work for you depends on what you want to know. They do have useful info on micelle sizes (aggregation #s, cmc's etc), but these are values for micelles alone, either in water or 0.1 M salt or something like that. If your question is how much detergent is actually associated with a particular protein you'll unfortunately have to measure that yourself (see Michaels message), since this will be protein and condition dependent (ie a large membrane protein will have more detergent associated with it than a small one). There was a thread on this subject on this board a couple weeks back i think. Cheers, Bert Bert van den Berg University of Massachusetts Medical School Program in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: [EMAIL PROTECTED] http://www.umassmed.edu/pmm/faculty/vandenberg.cfm
________________________________ From: CCP4 bulletin board on behalf of Savvas Savvides Sent: Tue 11/20/2007 3:53 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Protein-detergent micelle sizes Hi Jacob, the Calbiochem and Anatrace product catalogues will do the trick for you. best wishes Savvas ---- Savvas N. Savvides L-ProBE, Unit for Structural Biology Ghent University K.L. Ledeganckstraat 35 9000 Ghent, BELGIUM Phone: +32-(0)9-264.51.24 ; +32-(0)472-92.85.19 Email: [EMAIL PROTECTED] http://www.eiwitbiochemie.ugent.be/units_en/structbio_en.html -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jacob Keller Sent: Tuesday, November 20, 2007 12:25 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Protein-detergent micelle sizes Dear Crystallographers, I am trying to gather literature values on the size of the detergent/micelle belts on solublized membrane proteins. Does anybody know of a good repository of this info, whether as a database or as a review paper, or otherwise? The rough values of 15-25kD to 35-50kD were given in one recent BB posting, but I was looking for a more systematic tabulation... Thanks, Jacob Keller ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.467.4049 cel: 773.608.9185 email: [EMAIL PROTECTED] *******************************************