There is also the soluble KcsA. Computational design of water-soluble analogues of the potassium channel KcsA. A. M. Slovic, H. Kono, J. D. Lear, J. G. Saven, and W. F. DeGrado (2004) PNAS 101, 1828-1833
Bil ************************************ Bil Clemons, PhD Assistant Professor of Biochemistry Caltech 157 Broad Center MC 114-96 Pasadena, CA 91125 (626) 395-1796 [EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]> ************************************ > From: Thomas J Magliery PhD <[EMAIL PROTECTED]> > Reply-To: Thomas J Magliery PhD <[EMAIL PROTECTED]> > Date: Mon, 3 Dec 2007 16:50:03 -0500 > To: <CCP4BB@JISCMAIL.AC.UK> > Subject: Re: [ccp4bb] how to change a membrane protein into a water soluble > protein? > > It's hard. See: > > J Mol Biol. 2005 May 6;348(3):777-87. > X-ray structure of a water-soluble analog of the membrane protein > phospholamban: > sequence determinants defining the topology of tetrameric and pentameric > coiled > coils. > Slovic AM, Stayrook SE, North B, Degrado WF. > > Slovic, A. M., Summa, C. M., Lear, J. D. & DeGrado, > W. F. (2002). Computational design of a water-soluble > analog of phospholamban. Protein Sci. 12, 337348. > > Li, H., Cocco, M. J., Steitz, T. A. & Engelman, D. E. > (2001). Conversion of phospholamban into a soluble > pentameric helical bundle. Biochemistry, 40, > 66366645. > > Frank, S., Kammerer, R. A., Hellstern, S., Pegoraro, S., > Stetefeld, J., Lustig, A. et al. (2000). Toward a high resolution > structure of phospholamban: design of > soluble transmembrane domain mutants. > Biochemistry, 39, 68256831. > > Tom > > > Daniel Jin wrote: >> Hi, >> I am wondering whether there is a way to turn a membrane protein with >> known crystal structure into a water soluble protein by systematic >> mutagenesis. I guess it should be doable if we introduce enough >> hydrophilic residues on the surface. Has anyone tested this crazy idea >> before? Thank you for your help. >> Best, >> Chen >> >> ------------------------------------------------------------------------ >> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try >> it now. >> <http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8H >> DtDypao8Wcj9tAcJ%20> > > > -- > Thomas J. Magliery, Ph.D. > Assistant Professor > Department of Chemistry > & Department of Biochemistry > The Ohio State University > 1043 Evans Laboratory > 100 West 18th Ave. > Columbus, OH 43210-1185 > > (614) 247-8425 office > (614) 292-1685 fax > [EMAIL PROTECTED] > http://www.chemistry.ohio-state.edu/~magliery >
