I'm trying to engineer a disulfide bond into a protein that has several other cysteines.
My question is whether there is a crystallization friendly reducing agent that can be used to prevent oxidation of the free cysteines without breaking the disulfide? Also, can I expect 100% disulfide formation from standard bacterial expression (assuming good geometry of the cysteines)? Thanks, Kendall -- Kendall W. Nettles, PhD Assistant Professor Department of Cancer Biology The Scripps Research Institute 5353 Parkside Dr. Jupiter Fl 33458
