Dear all, a question very related to the discussion before. I have been reading the papers about the thermofluor experiment with great interest.
I wonder what people think about the underlying principles/ideas and the success that the method yielded in their own labs for crystallization or related purposes? Has anybody used this method with membrane proteins in order to find out the stability of the protein in the presence of a second detergent? Is the method limited to this certain dye (sypro orange)? Have a nice day Kornelius P.S.: I will make a summary of all opinions. ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349
