---------- Forwarded message ---------- From: Jayashankar <[EMAIL PROTECTED]> Date: Tue, May 20, 2008 at 7:34 PM Subject: Right terminal residues for constructs. To: CCP4BB@jiscmail.ac.uk
Dear friends and scientists, (A pre-Structural biological question.) I Have a multidomain protein , I know the domain boundaries, But am still not that rational to what residues a construct should start or end? But I have learned from people that changing one residue changes the fate of the construct. I will be greatful for the insights from the scientific community. I dont want to do any Directed evolution approach. -- S.Jayashankar Research Student Institute for Biophysical Chemistry Hannover Medical School Germany
